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Series GSE70756 Query DataSets for GSE70756
Status Public on May 09, 2016
Title Embryo thermal manipulation: muscle transcriptional profile in chickens submitted or not to heat challenge 34d posthach
Organism Gallus gallus
Experiment type Expression profiling by array
Summary Meat type chickens have limited capacities to cope with high environmental temperatures, sometimes leading to important mortality in farms and subsequent economic loss. One of the strategies to increase the sustainability of broiler chicken production is to enhance their adaptive capacities to face heat exposure using heat conditioning (C) during embryogenesis. This strategy was shown to improve thermotolerance in later life. The aim of this study was to determine the effect of C (39.5°C, 12h/24 vs 37.8°C from d7 to d16 of embryogenesis) and of a subsequent heat challenge (32°C for 5h, CH) applied on d34, on global gene expression in the Pectoralis major muscle (PM). Chicken Genome Array (60 K) was used to compare muscle gene expression profiles of Control (T) and heat-conditioned C chickens (characterized by low body temperature) reared at 21°C, and reared at 32°C (TCH and CCH respectively) in a dye-swap design with four comparisons and 8 broilers per condition. Real-time RT-PCR was subsequently performed to validate differential expressions in each comparison. Gene ontology, clustering and network building strategies were used. This study was performed with the financial support of the French Agence Nationale de la Recherche, Project «Jeunes Chercheuses et Jeunes Chercheurs», ANR-09-JCJC-0015-01, THERMOCHICK.
 
Overall design Four-condition experiment on chicken breast muscle samples, Controls at 32°C (TCH) vs. Controls at 21°C (T), heat-conditioned at 21°C (C) vs. Controls at 21°C (T), heat-conditioned at 32°C (CCH) vs. heat-conditioned at 21°C (C), heat-conditioned at 32°C (CCH) vs. Controls at 32°C (TCH). Biological replicates: 8 per treatment characterized by low body temperatures in C and CCH treatments and high body temperatures in T and TCH treatments.
 
Contributor(s) Loyau T, Hennequet-Antier C, Leduc M, Crochet S, Sannier M, Coustham V, Duclos MJ, Berri C, Mignon-Grasteau S, Tesseraud S, Brionne A, Métayer-Coustard S, Moroldo M, Lecardonnel J, Martin P, Lagarrigue S, Yahav S, Collin A
Citation(s) 27142519
Submission date Jul 10, 2015
Last update date May 11, 2016
Contact name Anne Collin
E-mail(s) Anne.Collin@tours.inra.fr
Organization name INRA
Lab URA
Street address UR83 Recherches Avicoles
City Nouzilly
ZIP/Postal code F-37380
Country France
 
Platforms (1)
GPL20679 Agilent-033524 gallus_exp_microarray_SLagarrigue_8x60k_V0_fev2011
Samples (64)
GSM1817356 Control-21°C T6 vs. Heat-conditioned-21°C C6
GSM1817357 Heat-conditioned-32°C CCH5 vs. Control-32°C TCH5
GSM1817358 Heat-conditioned-21°C C3 vs. Heat-conditioned-32°C CCH3
Relations
BioProject PRJNA289503

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70756_RAW.tar 1.3 Gb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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