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Series GSE70679 Query DataSets for GSE70679
Status Public on Jul 10, 2015
Title Prostate Cancer Risk SNPs in Androgen Receptor Target Sites
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We profiled androgen receptor (AR) genomic targets using high-throughput sequencing of chromatin-immunoprecipitated (ChIP) DNA from TMPRSS2-ERG fusion gene positive DUCaP prostate cancer cells. ChIp-seq and microarray gene expression profiling datasets were integrated with the NHGRI GWAS PCa risk SNPs catalog to identify disease susceptibility SNPs localized within functional androgen receptor binding sites (ARBSs). Eighty GWAS index or linked SNPs were found to be localized in ARBSs. Among these rs11891426:T>G in the 7th intron of the melanophilin gene was found located within a novel putative auxiliary AR binding motif, which we found enriched in the neighborhood of canonical androgen responsive elements. T→G exchange attenuated the transcriptional activity of the ARBS in an AR reporter gene assay of prostate cancer cell models. It went also in line with decreased melanophilin protein level in primary prostate tumors with G allele.These results unravel a hidden link between androgen receptor and a functional PCa risk SNP, whose allele alteration affects androgen regulation of its host gene melanophilin .
Overall design Genomic profile of androgen receptor binding sites of androgen or vehicle treated DUCaP cells using ChIP-seq. IgG precipiated DNAs from both treatments served as controls.
Contributor(s) Klocker H
Citation(s) 26411452
Submission date Jul 09, 2015
Last update date May 15, 2019
Contact name Klocker A Helmut
Organization name Medical University of Innsbruck
Department Department of Urology
Street address Anichstrasse 35
City Innsbruck
ZIP/Postal code A-6020
Country Austria
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (4)
GSM1816381 AR_vehicle_ChIPseq
GSM1816382 AR_androgen_ChIPseq
GSM1816383 IgG_vehicle_ChIPseq
BioProject PRJNA289344
SRA SRP060602

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Supplementary file Size Download File type/resource
GSE70679_AR_A_vs_AR_NA_peaks.txt.gz 808.2 Kb (ftp)(http) TXT
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