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| Status |
Public on Mar 20, 2016 |
| Title |
Taf4 is required for preinitiation complex formation at genes associated with neuronal differentiation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Occupancies of promoters by TBP, PolII, TFIIB as well as chromatin marks H3K4Me3 and H3K27ac were compared in embryonic stem cells (ESC) and in embryoid bodies (EB) treated with retinoic acid (RA) derived from the WT and Taf4a-/- lineages. Reduced occupancies for these factors and decreased signals for H3K4Me3 and H3K27ac were observed for genes associated with neuronal differentiation in EB of Taf4a-/- line compared to EB of WT line.
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| Overall design |
ChIP-seq experiment was performed for TFIIB, Pol II, H3K4me3, H3K27ac and TBP in wt and TAF4-/- mouse embryonic stem cells (ESC) and embryoid bodies (EB) treated with retinoic acid (RA).
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| |
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| Contributor(s) |
Martianov I, Langer D, Davidson I |
| Citation(s) |
27026076 |
| |
| Submission date |
Jul 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Igor Martianov |
| E-mail(s) |
martiano@igbmc.fr
|
| Phone |
0033388653440
|
| Organization name |
IGBMC
|
| Street address |
1 rue Laurent Fries
|
| City |
Illkirch |
| ZIP/Postal code |
67400 |
| Country |
France |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA289302 |
| SRA |
SRP060559 |
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