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| Status |
Public on Jul 07, 2015 |
| Title |
Transcribed ultraconserved region 339 promotes carcinogenesis by sequestering tumor suppressor microRNAs |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The transcribed ultraconserved regions (T-UCRs) are a family of long non-coding RNAs implicated in human carcinogenesis. The mechanism of action of T-UCRs and the factors regulating their expression in human cancers are poorly understood. In this study we show that high expression of uc.339 correlates with lower survival in 204 non-small cell lung cancer (NSCLC) patients. We also show that uc.339 found up-regulated in archival NSCLC samples, functions as a decoy for miR-339-3p, -663-3p and -95-5p. As a result, Cyclin E2, a direct target of these microRNAs is up-regulated, promoting cancer growth and migration. We provide evidence from cell lines and primary samples suggesting that TP53 directly regulates uc.339. Our results support a key role for uc.339 in lung cancer.
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| Overall design |
4 experimental groups in triplicate (total: 12 samples). A339= A549 infected with a lentivirus over-expressing uc.339; AE= A549 infected with an empty lentivirus (control of A339); L339= LoVo infected with a lentivirus over-expressing uc.330; LE= LoVo infected with an empty lentivirus (control of L339). 1-2-3 refer to each experiment.
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| Contributor(s) |
Vannini I, Challagundla KB, Plousiou M, Bandini E, Fanini F, Paliaga G, Crawford M, Ferracin M, Davuluri RV, Guo Z, Cortez M, Ivan C, Zhang X, Fernandez-Cymering C, Han L, Wise PM, Carloni S, Ling H, Murtadha M, Gitlitz BJ, Laird-Offringa IA, Nana-Sinkam P, Negrini M, Liang H, Amadori D, Cimmino A, Calin GA, Fabbri M |
| Citation missing |
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| Submission date |
Jul 06, 2015 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Muller Fabbri |
| E-mail(s) |
mfabbri@chla.usc.edu
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| Organization name |
University of South California - Children's hospital Los Angeles
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| Street address |
4650 Sunset Blvd
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| City |
Los Angeles |
| ZIP/Postal code |
90027 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA288971 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE70540_RAW.tar |
56.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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