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| Status |
Public on Jul 01, 2015 |
| Title |
Transcriptomic profiles of M. barkeri Fusaro DSMZ804 and Pyr+ strains |
| Organism |
Methanosarcina barkeri str. Fusaro |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this work was to elucidate the mechanism by which pyruvate is utilized as a substrate in a mutant strain of Methanosarcina barkeri Fusaro. In this study, using RNAseq we gained insight into how the mutant strain modulate its transcriptional profile in order to use pyruvate as a substrate. In addition, we obtained information on how methanogens respond to pyruvate at the transcriptional level.
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| Overall design |
The mRNA from of Methanosarcina barkeri Fusaro DSMZ804 and Pyr+ strains grown on a variety of substrates (methanol, acetate, methanol-acetate, methanol-pyruvate, methanol-pyruvate-acetate) were harvested sequenced and mapped to M. barkeri genome. Pairwise comparisons between two cell lines of the Pyr+ strain and the DSMZ 804 strain were performed in all substrates tested.
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| Contributor(s) |
López Muñoz MM, Metcalf WW |
| Citation(s) |
26350133 |
| |
| Submission date |
Jun 29, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
William Metcalf |
| Organization name |
University of Illinois at Urbana Champaign
|
| Street address |
601 S Goodwin Ave
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
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| Platforms (1) |
| GPL20638 |
Illumina HiSeq 2000 (Methanosarcina barkeri str. Fusaro) |
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| Samples (54)
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| Relations |
| BioProject |
PRJNA288456 |
| SRA |
SRP059944 |
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