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Series GSE70346 Query DataSets for GSE70346
Status Public on May 13, 2016
Title Genome-wide analysis of Estrogen Receptor-mediated response to acute Estradiol treatment in liver (ChIP-seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Estrogen-based treatments have numerous extra-reproductive effects. On the one hand, they have protective metabolic actions against abdominal fat accumulation, type 2 diabetes, liver steatosis,… But, on the other hand, the oral route of administration, presumably due to a hepatic impact on liver-derived coagulation factors, appears to increase risks of venous thrombosis and pulmonary embolism. The characterization of liver genes expression regulations by these hormones thereby appears of utmost interest, but the estrogen-sensitive transcriptome of liver and ER cistrome was so far explored under chronic hormonal treatment. To explore the early steps of these mechanisms, we determined here the short-term changes in liver transcriptional responses to acute E2 administration, and aimed to characterize the mechanisms allowing these programs to be engaged. Collectively, through the comparison of mice expressing or not ER, our data demonstrate that acute administration of E2 provokes genes expression variations which fit with a crucial role of ER in the prevention of liver steatosis in mice fed with a high fat diet by E2. They also evidenced higher proportion of ER binding sites (BSs) located at the direct vicinity (distance <3kb) of regulated genes than what is determined in human cancer cell models. Besides this specific aspect of ER cistrome in vivo, we unexpectedly found that 40 % of the BSs of the pioneer factor Foxa2 were dependent upon the expression of ER that indirectly influences the distribution of the nucleosomes harbouring a dimethylated H3K4 around these Foxa2 sites.
 
Overall design Whole-genome analysis of estrogen receptor (ER) and FOXA2 binding events associated with the cartography of two histone marks (H3K4me2 and H3K27ac) in livers from wild-type or ERKO mice.
 
Contributor(s) Palierne G, Fabre A, Solinhac R, Le Péron C, Avner S, Lenfant F, Fontaine C, Salbert G, Flouriot G, Arnal JF, Métivier R
Citation(s) 27164166
Submission date Jun 26, 2015
Last update date May 15, 2019
Contact name Raphaël Métivier
E-mail(s) raphael.metivier@univ-rennes1.fr
Organization name CNRS UMR 6290
Lab SP@RTE
Street address Campus de Beaulieu
City Rennes
ZIP/Postal code 35042 Cedex
Country France
 
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (12)
GSM1724230 ERWT_Input 1
GSM1724231 ERWT_Input 2
GSM1724232 ERWT_ER_P
This SubSeries is part of SuperSeries:
GSE70350 Genome-wide analysis of Estrogen Receptor-mediated response to acute Estradiol treatment in liver
Relations
BioProject PRJNA288344
SRA SRP059936

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70346_RAW.tar 2.5 Gb (http)(custom) TAR (of WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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