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Series GSE70220 Query DataSets for GSE70220
Status Public on Jun 25, 2015
Title Human Calu-3 cell transcriptome response to human interferon α [miRNA]
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The purpose is to obtain samples for mRNA and miRNA analysis with and without interferon α treatment; in parallel, to obtain cell pellets to forward to PNNL for use in top-down proteomics pilot experiments
 
Overall design Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in quadruplet; time-matched mocks were collected in quadruplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection
 
Contributor(s) Eisfeld AJ, Stratton K, Waters KM, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 24, 2015
Last update date Aug 31, 2016
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL19730 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
Samples (23)
GSM1720956 IFNaCL001_Mock_6h_1_microRNA
GSM1720957 IFNaCL001_Mock_6h_2_microRNA
GSM1720958 IFNaCL001_Mock_6h_3_microRNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA287910

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70220_RAW.tar 77.0 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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