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Series GSE70217 Query DataSets for GSE70217
Status Public on Jun 25, 2015
Title Human Calu-3 cell transcriptome response to human interferon α [mRNA]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose is to obtain samples for mRNA and miRNA analysis with and without interferon α treatment; in parallel, to obtain cell pellets to forward to PNNL for use in top-down proteomics pilot experiments
 
Overall design Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in quadruplet; time-matched mocks were collected in quadruplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection
 
Contributor(s) Eisfeld AJ, Stratton K, Waters KM, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 24, 2015
Last update date Jan 09, 2018
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (23)
GSM1720909 IFNaCL001_Mock_6h_1_RNA
GSM1720910 IFNaCL001_Mock_6h_2_RNA
GSM1720911 IFNaCL001_Mock_6h_3_RNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA287907

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70217_RAW.tar 49.6 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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