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Series GSE70161 Query DataSets for GSE70161
Status Public on Jul 18, 2016
Title Chromatin targets of androgen receptor and PIAS1 in molecular apocrine breast cancer cells [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The majority of breast cancer subtypes express androgen receptor (AR) in addition to estrogen receptor a (ERa). Depending on the breast cancer subtype androgen signaling has either stimulatory or inhibitory roles in breast cancer cell growth. We have mapped AR cistrome in ERa negative human molecular apocrine breast cancer MDA-MB453 cells and analyzed it in relation to the androgen-regulated transcriptome in the same cells. We have also examined the effect of silencing of the coregulator SUMO ligase PIAS1 on the androgen-regulated transcriptome and AR cistrome in MDA-MB453 cells. Our results show that the MDA-MB453 cells share with VCaP prostate cancer cells a core AR cistrome and target gene signature linked to cancer cell growth and that PIAS1 acts as an AR target gene-selective coregulator in MDA-MB453 cells.
 
Overall design All ChIP-seq samples were collected from cells treated with 10 nM R1881 (synthetic androgen methyltrienolone) for 2 h. In addition, AR ChIP-seq samples were collected from cells exposed to control siRNA (siNON, Dharmacon Non-targeting pool) or PIAS1 siRNA (siPIAS1, Dharmacon ON-TARGETplus SMARTpool for human PIAS1). IgG sample was collected from siNON and siPIAS1 cells and used as background control. Biological duplicate samples of the AR, PIAS1 and single IgG ChIP-seq samples were analyzed by using Solexa/Illumina 1.5.
 
Contributor(s) Malinen M, Toropainen S, Jääskeläinen T, Rytinki M, Sahu B, Jänne OA, Palvimo JJ
Citation(s) 26219822
Submission date Jun 23, 2015
Last update date May 15, 2019
Contact name Sari Toropainen
E-mail(s) sari.toropainen@uef.fi
Organization name University of Eastern Finland
Department School of Medicine
Lab Biomedicine
Street address Yliopistonranta 1 E
City Kuopio
ZIP/Postal code 70211
Country Finland
 
Platforms (1)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
Samples (7)
GSM1717859 MDA-MB453_IgG
GSM1717860 MDA-MB453_AR_siNON_R1881_rep1
GSM1717861 MDA-MB453_AR_siNON_R1881_rep2
This SubSeries is part of SuperSeries:
GSE70163 Chromatin targets of androgen receptor and coregulator PIAS1 in molecular apocrine breast cancer cells
Relations
BioProject PRJNA287765
SRA SRP059781

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Supplementary file Size Download File type/resource
GSE70161_MB453_AR_siNON_rep1+rep2_peaks.bed.gz 215.9 Kb (ftp)(http) BED
GSE70161_MB453_AR_siPIAS1_rep1+rep2_peaks.bed.gz 314.9 Kb (ftp)(http) BED
GSE70161_MB453_PIAS1_rep1+rep2_peaks.bed.gz 8.6 Kb (ftp)(http) BED
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Processed data are available on Series record

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