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Series GSE70048 Query DataSets for GSE70048
Status Public on Jun 20, 2015
Title caArray_green-00030: 2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling
Organism Mus musculus
Experiment type Expression profiling by array
Summary 2-methoxyestradiol (2ME2) induces mammary gland differentiation through amphiregulin-EGFR mediated signaling: molecular distinctions from the mammary gland of pregnant mice.High levels of 2ME2 are observed in the late stages of pregnancy. We investigated the role of 2ME2 on normal mammary gland development. Large scale gene expression assays were performed using Affymetrix GeneChips in pursuit of detailed molecular basis. (1) Mammary glands of wild type FVB mice administered 75 or 150 mg/kg of 2ME2 (2) Mammary glands of normal FVB/Nj mice (i) at day 16 of pregnancy, (ii) day 2 of lactation (iii) day 30 of post-lactation, and (3) mammary epithelial SCp2 cells after 6, 24 and 48 hours of 10 micromol 2ME2 treatment were examined. In vivo studies revealed that 2ME2 treatment up regulates the expression of amphiregulin. The clue to the role of 2ME2 in differentiation comes from studies in vitro which detected down regulation of inhibitor of differentiation (Id-1) gene and consequent up regulation of amphiregulin. The differentiation of E2 negative SCp2 cells by 2ME2 indicate estradiol independent mechanism. For details, please see our paper in Endocrinology 2006.
**NOTE: Migrated from caArray 1.x, identifier='gov.nih.nci.ncicb.caarray:Experiment:1015897590892008:1'
 
Overall design green-00030
Assay Type: Gene Expression
Provider: Affymetrix
Array Designs: mg_u74av2, Mouse430_2
Organism: Mus musculus (ncbitax)
Tissue Sites: murine mammary gland
Material Types: cell, synthetic_RNA, organism_part, total_RNA
 
Citation(s) 17158205
Submission date Jun 19, 2015
Last update date Feb 11, 2019
Contact name Mervi Heiskanen
E-mail mervi.heiskanen@nih.gov
Phone 2402765175
Organization name NCI
Department CBIIT
Street address 9609 Medical Center Drive, 1W378
City Rockville
State/province MD
ZIP/Postal code 20850
Country USA
 
Platforms (2)
GPL81 [MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (55)
GSM1716274 green-00030: JG2ME0mgA
GSM1716275 green-00030: JG2ME0mgB
GSM1716276 green-00030: JG2ME0mgC
Relations
BioProject PRJNA287539

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE70048_RAW.tar 181.5 Mb (http)(custom) TAR (of CEL)
GSE70048_caArray_green-00030.tar.gz 7.0 Kb (ftp)(http) TAR
GSE70048_prg2_4A_r2_upload.xls.gz 212.6 Kb (ftp)(http) XLS
GSE70048_prg2_4B_r2_upload.xls.gz 385.3 Kb (ftp)(http) XLS
Raw data provided as supplementary file
Processed data is available on Series record

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