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| Status |
Public on Oct 05, 2016 |
| Title |
Function of deubiquitinase USP21 in mouse embryonic stem cell [RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Ubiquitination-mediated protein degradation of key transcriptional factors is important to the self-renewal of embryonic stem (ES) cells. However, little is known about the deubiquitination in ES self-renewal and differentiation. Here, we report that deubiquitinase USP21 is an important positive regulator to keep ES cells under undifferentiation stasus by deubiquitination and stabilization of Nanog, a key transcriptional factor of ES cells. Loss of USP21 led to ES cells differentiation and defect in reprogramming.
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| Overall design |
Gene expression profiles of mouse embryonic stem cell after USP21 knockdown were generated by deep sequencing, using Illumina HighSeq2000, respectively.
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| Contributor(s) |
Wang P, Xie X, Jiang C, Jin J, Liu Z |
| Citation(s) |
27886188 |
| |
| Submission date |
Jun 18, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Liu Zhenping |
| E-mail(s) |
093256@tongji.edu.cn
|
| Organization name |
Tongji University
|
| Street address |
1239 Si Ping Road
|
| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE79891 |
Function of deubiquitinase USP21 in mouse embryonic stem cell |
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| Relations |
| BioProject |
PRJNA287352 |
| SRA |
SRP059630 |
