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Series GSE69943 Query DataSets for GSE69943
Status Public on Jun 18, 2015
Title IHH microRNA response to genetically-reconstructed Zaire Ebola viruses [miRNA]
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants:
Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976
Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein.
Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP).
Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins.
 
Overall design Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 6, 12, 24, 48, and 72 hrs post infection in triplicate.
 
Contributor(s) Kawaoka Y, Waters KM, Halfmann P, Eisfeld AJ
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 17, 2015
Last update date Aug 31, 2016
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL19730 Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version)
Samples (15)
GSM1713603 EIHH002_Mock_12hr_1_microRNA
GSM1713604 EIHH002_Mock_12hr_2_microRNA
GSM1713605 EIHH002_Mock_12hr_3_microRNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA287216

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69943_RAW.tar 50.9 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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