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Series GSE69942 Query DataSets for GSE69942
Status Public on Jun 18, 2015
Title IHH mRNA response to genetically-reconstructed Zaire Ebola viruses [mRNA]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants:
Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976
Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein.
Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP).
Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins.
 
Overall design Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 6, 12, 24, 48, and 72 hrs post infection in triplicate.
 
Contributor(s) Kawaoka Y, Waters KM, Halfmann P, Eisfeld AJ
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 17, 2015
Last update date Jan 09, 2018
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL13497 Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version)
Samples (84)
GSM1713519 EIHH002_Mock_0hr_1_RNA
GSM1713520 EIHH002_Mock_0hr_2_RNA
GSM1713521 EIHH002_Mock_0hr_3_RNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA287217

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69942_RAW.tar 180.7 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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