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Series GSE69924 Query DataSets for GSE69924
Status Public on Aug 23, 2015
Title Direct reprogramming of mouse fibroblasts into cardiomyocytes with chemical cocktails
Organism Mus musculus
Experiment type Expression profiling by array
Summary The direct conversion, or trans-differentiation, of non-cardiac cells into cardiomyocytes by forced expression of transcription factors and microRNAs provide promising ways of cardiac regeneration. However, genetic manipulations are still not desirable in real clinical applications. we report the generation of automatically beating cardiomyocyte-like cells from mouse fibroblasts with only chemical cocktails. These chemical-induced cardiomyocyte-like cells (CiCMs) express cardiomyocyte-specific markers, exhibit sarcomeric organization, and possess typical cardiac calcium flux and electrophysiological features. Microarray-bassed gene expression patterns of Mouse embryonic fibroblasts (MEFs), CiCMs, and cardiomyocytes(CMs) indicated a clear transition from dividing MEFs to differentiated cardiomyocyte-like state in CiCM samples.
Overall design Mouse embryonic fibroblasts were treated with a small-molecule combination CRFVPT (10 μM CHIR99021 (C); 10 μM RepSox (R); 50 μM Forskolin (F); 0.5 mM VPA (V); 5 μM Parnate, (P); 1 μM TTNPB (T)) to induce transdifferentiation to chemical-induced cardiomyocyte-like cells. CiCMs beating clusters were picked at day 24 for analysis. MEFs were isolated from mouse embryos, and CMs were isolated from mouse hearts. Total RNA of MEFs, CiCMs and CMs were extracted and hybridization on Affymetrix microarrays.
Contributor(s) Fu Y, Huang C, Xu X, Gu H, Ye Y, Jiang C, Qiu Z, Xie X
Citation(s) 26292833
Submission date Jun 16, 2015
Last update date Feb 11, 2019
Contact name Yanbin Fu
Phone +86-15021381429
Organization name Tongji University
Lab Laboratory of Receptor-based Bio-medicine
Street address No.1239, SiPing Road
City Shanghai
ZIP/Postal code 200092
Country China
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (9)
GSM1712851 MEFs, biological rep1
GSM1712852 MEFs, biological rep2
GSM1712853 MEFs, biological rep3
BioProject PRJNA287135

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Supplementary file Size Download File type/resource
GSE69924_RAW.tar 32.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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