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Series GSE69837 Query DataSets for GSE69837
Status Public on Feb 04, 2016
Title Inverse relationship between microRNA-155 and -184 expression with increasing conjunctival inflammation during ocular Chlamydia trachomatis infection
Organism Homo sapiens
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Trachoma, a preventable blinding eye disease, is initiated by ocular infection with Chlamydia trachomatis (Ct). MicroRNA (miR) are post-transcriptional regulators of gene expression and play a major role in health and disease. We have investigated the miR profile during C. trachomatis infection of epithelial cells in vitro and in vivo during follicular trachoma with current C. trachomatis infection. Small RNA sequencing was carried out on human epithelial cells infected in vitro and on samples from five children with follicular trachoma with current Ct infection and five children with no evidence of clinical trachoma or infection. In vitro two strains of ocular Ct that differ in virulence, A2497 and isogenic plasmid-free A2497 were used to infect epithelial cell lines. RNAseq results were confirmed by qPCR in six in vitro biological replicates and in 163 clinical samples. Differential miR expression was not detected in isolated epithelial cells infected in vitro at 48 hours post infection. HCjE cells, a conjunctival epithelial cell line, have markedly different miR background expression compared to Hep2 cells. The differing miR profiles of Hep2c and HCjE suggest caution should be used when extrapolating data from Hep2 cells to a tissue-specific clinical scenario. In follicular trachoma, miR-155, miR-150, miR-142, miR-181b, miR-181a, miR-342 and miR-132 were up-regulated during current Ct infection. MiR-4728 and miR-184 were down-regulated in follicular trachoma independent of Ct infection. In follicular trachoma, miR expression reflects development and regulation of the immune response during current Ct infection and a prolonged period of wound healing following Ct clearance.
 
Overall design (1) miRNA sequencing of conjunctival swab samples from five individuals with trachomatous disease and five normal healthy control individuals. (2) miRNA sequencing of two cell lines (HCjE and Hep2) with three biological repeats of each of the following conditions: mock-infected, Chlamydia trachomatis strain A2497P- infected, and Chlamydia trachomatis strain A2497 infected
 
Contributor(s) Derrick T, Last AR, Burr SE, Roberts CH, Nabicassa M, Cassama E, Bailey RL, Mabey DC, Burton MJ, Holland MJ
Citation(s) 26842862
Submission date Jun 12, 2015
Last update date Jan 25, 2019
Contact name Tamsyn Ruth Derrick
E-mail tamsyn.derrick@lshtm.ac.uk
Phone 02079272419
Organization name london school of hygiene and tropical medicine
Street address keppel street
City london
ZIP/Postal code WC1E 7HT
Country United Kingdom
 
Platforms (1)
GPL15520 Illumina MiSeq (Homo sapiens)
Samples (28)
GSM1709834 Follicular trachoma case 5
GSM1709835 Follicular trachoma case 7
GSM1709836 Follicular trachoma case 19
Relations
BioProject PRJNA286886
SRA SRP059444

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69837_miRNA_readcounts.txt.gz 28.7 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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