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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 17, 2016 |
| Title |
A fully validated blood stem/progenitor cell regulatory network reveals mechanisms of cell state stabilisation |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The aim of this study was to determine the genomic binding sites of important haematopoietic transcription factors in haematopoietic cell lines.
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| Overall design |
416Bs cells were fixed with either 1 or 0.4 % formaldehyde for 10 mins. Chromatin was isolated, sonicated for 7 mins (30 sec on, 30 sec off), and specific antibodies were used to pull down the transcription factors of interest after a pre-clearing step. Chromatin was washed, de-crosslinked, amplified, size selected by gel purification and sequenced.
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| Contributor(s) |
Schütte J, Wang H, Antoniou S, Jarrett A, Wilson NK, Riepsaame J, Calero-Nieto FJ, Basilico S, Kinston SJ, Hannah R, Chan MC, Nurnberg ST, Ouwehand WH, Bonzanni N, de Bruijn MF, Göttgens B |
| Citation(s) |
26901438 |
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| Submission date |
Jun 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Bertie Gottgens |
| E-mail(s) |
bg200@cam.ac.uk, nkw22@cam.ac.uk
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| Organization name |
University of Cambridge
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| Street address |
Jeffrey Cheah Biomedical Centre
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA286742 |
| SRA |
SRP059367 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69776_RAW.tar |
1.2 Gb |
(http)(custom) |
TAR (of BED, BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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