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| Status |
Public on Feb 13, 2017 |
| Title |
Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Yin Yang 1 (YY1) is a multifunctional transcription factor shown to be critical in a variety of biological processes. Although it has been reported to be regulated by multiple types of post-translational modifications (PTMs), whether YY1 is methylated, which enzyme methylates YY1, and hence the functional significance of YY1 methylation remain completely unknown. Here we reported the first methyltransferase, SET7/9 (KMT7), capable of methylating YY1 in vitro and in vivo at two highly conserved lysine (K) residues, K173 and K411, located in two distinct domains, one in the central glycine-rich region and the other in the very carboxyl-terminus. Functional study revealed that SET7/9-mediated YY1 methylation regulated YY1 DNA-binding activity both in vitro and at specific genomic loci in cultured cells. Consistently, SET7/9-mediated YY1 methylation was shown to involve in YY1-regulated gene transcription and cell proliferation. Our findings revealed a novel regulatory strategy, methylation by lysine methyltransferase, imposed on YY1 protein, and linked YY1 methylation with its biological functions.
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| Overall design |
The YY1 ChIP-seq in this study was designed to reveal YY1 genomic binding sites in HeLa cells.
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| Contributor(s) |
Liu W, Zhang W |
| Citation(s) |
26902152 |
| |
| Submission date |
Jun 10, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Wenjuan Zhang |
| Organization name |
Gannan Medical University
|
| Department |
First Affiliated Hospital
|
| Lab |
Department of Laboratory Medicine
|
| Street address |
No. 23, Qingnian Road
|
| City |
Ganzhou |
| State/province |
Jiangxi |
| ZIP/Postal code |
341000 |
| Country |
China |
| |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE69759 |
Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation |
|
| Relations |
| BioProject |
PRJNA286701 |
| SRA |
SRP059340 |
