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Series GSE69405 Query DataSets for GSE69405
Status Public on Jun 03, 2015
Title Single-cell RNA sequencing of lung adenocarcinoma patient-derived cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Other
Summary To address how intratumoral heterogeneity affects anti-cancer drug responses, we profiled transcriptomes of single cancer cells originating from lung adenocarcinoma patient-derived xenograft (PDX) tumors.
 
Overall design We performed single-cell RNA sequencing (scRNA-Seq) together with bulk sequencing by applying Smart-Seq protocol (Ramsk├Âld et al., Nat Biotechnol 2012). Enrichment of cancer cells in PDX from primary tumor (LC-PT-45: bulk RNA-Seq, n=1) was identified by histopathological examination and genomic signatures. Tumor cell-enriched PDX cells (LC-PT-45: scRNA-Seq, n=34; bulk RNA-Seq, n=9) were analyzed, and additional batch (LC-Pt-45-Re: scRNA-Seq, n=43; bulk RNA-Seq, n=7) was obtained to check comparable results. H358 human lung cancer cells (scRNA-Seq, n=50; bulk RNA-Seq, n=1) were used as cell line controls. Another lung cancer PDX case (LC-MBT-15: scRNA-Seq, n=49; bulk RNA-Seq, n=7) was prepared to validate our analytical strategy applied in the LC-PT-45 case.
 
Contributor(s) Kim K, Lee HW, Lee H, Joo KM, Park W
Citation(s) 26084335
Submission date May 31, 2015
Last update date May 15, 2019
Contact name Kyu-Tae Kim
Organization name Samsung Medical Center
Department Samsung Genome Institute
Street address Irwon-Ro 81
City Seoul
ZIP/Postal code 135-710
Country South Korea
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (208)
GSM1702587 H358_Pooled
GSM1702588 H358_SC05
GSM1702589 H358_SC09
Relations
BioProject PRJNA285793
SRA SRP059035

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Supplementary file Size Download File type/resource
GSE69405_PROCESSED_GENE_TPM_ALL.txt.gz 6.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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