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Series GSE6925 Query DataSets for GSE6925
Status Public on Sep 21, 2007
Title BW25113 ymgB and W/T in LBglu 24h biofilm cells
Platform organism Escherichia coli K-12
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary For the microarray experiments, 10 g glass wool (Corning Glass Works, Corning, N.Y.) were used to form biofilms (30) in 250 mL in 1 L Erlenmeyer shake flasks which were inoculated with overnight cultures diluted that were 1:167. The cells were shaken at 250 rpm and 37°C for 24 hours to form biofilms on the glass wool, and RNA was isolated from the suspension cells and the biofilm.
Keywords: E. coli biofilm
 
Overall design Strain: E. coli BW25113 wild type and ymgB mutant
Medium: LBglu
Cells: biofilm cells on glass wool
Time: 24h
 
Contributor(s) Lee J, Wood TK, Peti W, Contreras RG, Zhang X
Citation(s) 17765265
Submission date Jan 30, 2007
Last update date Mar 16, 2012
Contact name Jintae Lee
E-mail(s) Jintae.Lee@chemail.tamu.edu
Phone 1-979-845-1744
Fax 1-979-845-6446
Organization name Texas A&M University
Department Chemical Engineering
Street address MS 3122
City College Station
State/province TX
ZIP/Postal code 77843-3122
Country USA
 
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (2)
GSM159471 BW25113 W/T LBglu 24h biofilm cells
GSM159472 BW25113 ymgB LBglu 24h biofilm cells
Relations
BioProject PRJNA99349

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6925_RAW.tar 2.5 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file

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