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Series GSE69125 Query DataSets for GSE69125
Status Public on Mar 02, 2016
Title The effect of knockdown Abl kinases on breast cancer cells' global transcriptome
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To gain insight into the signaling pathway(s) required for ABL1/ABL2-dependent bone metastasis, we evaluated the consequences of single or double inactivation of ABL1 and ABL2 on the transcriptome of breast cancer cells. Double ABL1/ABL2 knockdown was required to decrease the levels of p-CrKL by more than 90%, indicative of inactivation of the endogenous ABL kinases. To examine the consequences of depleting the ABL kinases on the transcriptome of metastatic breast cancer cells we employed next generation sequencing (RNAseq) analysis. We found that 180 genes were significantly down-regulated and 40 genes were significantly up-regulated in ABL1/ABL2 knockdown cells.
 
Overall design Four samples were analyzed control, Abl single knockdown, Arg single knockdown, Abl/Arg double knockdown. Experiments were performed in triplicate.
 
Contributor(s) Wang J, Pendergast AM
Citation(s) 26838548
Submission date May 21, 2015
Last update date May 15, 2019
Contact name Jun Wang
Organization name Duke University
Department Pharmacology and Cancer Biology
Lab Pendergast lab
Street address 308 Research Drive LSRC C231
City Durham
State/province North Carolina
ZIP/Postal code 27710
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (4)
GSM1693176 Scr
GSM1693177 miAbl
GSM1693178 miArg
Relations
BioProject PRJNA284608
SRA SRP058587

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69125_10408_gene_exp.diff.gz 4.8 Mb (ftp)(http) DIFF
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Raw data are available in SRA
Processed data are available on Series record

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