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Series GSE69043 Query DataSets for GSE69043
Status Public on Jan 11, 2016
Title Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary AR is tightly regulated by many transcriptional cofactors, including key pioneer factor such as FOXA1 and GATA2. While FOXA1 was recently shown to be able to redistribute AR across the genome, how GATA2 regulates AR cistrome has not been carefully investigated. Here, we report that, unlike FOXA1, GATA2 is unable to reprogram AR, but instead it enhances AR program by inducing AR expression and augmenting AR co-occupancy, thereby acting as a pure AR coactivator, rather than a pioneer factor. On the other hand, AR co-occupancy enhances both GATA2 and FOXA1 binding on the chromatin, forming a positive feedback loop. Importantly, we found that FOXA1 is also capable of reprogramming GATA2 by recruiting GATA2 from GATA motif to FKHD-containing regions, being analogous to its pioneering effect on AR signaling. By contrast, GATA2 simply acts as a co-activator of FOXA1 with a lack of pioneering ability.
 
Overall design ChIP_Seq examination of AR, FoxA1 and GATA2 binding sites in LNCaP and DU145 cells
 
Contributor(s) Zhao J, Yu J
Citation(s) 26751772
Submission date May 19, 2015
Last update date May 15, 2019
Contact name Jindan Yu
E-mail(s) jindan-yu@northwestern.edu
Organization name Northwestern University
Department Division of Hematology/Oncology
Lab Jindan Yu's lab
Street address 303 E. Superior St., Lurie 5-117
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL15456 Illumina HiScanSQ (Homo sapiens)
Samples (28)
GSM1691138 LNCaP GIPZ RPMI+10%FBS AR ChIP m11
GSM1691139 LNCaP shFOXA1 RPMI+10%FBS AR ChIP m12
GSM1691140 LNCaP GIPZ phenolfree RPMI+5%FCS AR ChIP m13
This SubSeries is part of SuperSeries:
GSE69045 Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer
Relations
BioProject PRJNA284390
SRA SRP058498

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69043_RAW.tar 1.4 Gb (http)(custom) TAR (of BEDGRAPH, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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