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Series GSE68981 Query DataSets for GSE68981
Status Public on Dec 24, 2016
Title Single Cell Transcriptomics Reveals Multi-Dimensional Heterogeneity in Early Hematopoietic Precursors
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hematopoietic stem cells (HSCs) and multipotent progenitors (MPPs) are at the apex of the hematopoietic hierarchy. The relationship among HSC and MPPs, their heterogeneity and regulation are challenging subjects of continuing study. We performed single-cell RNA-seq on HSC and their immediate progeny MPP1 cells and compared results with bulk RNA-seq data from 18 hematopoietic populations, literature compilations of cell cycle-related genes, lineage specific genes, and putative quiescence genes. HSC and MPP1 were separated into several distinct subsets including actively cycling, quiescent and a putative hibernating subset. The quiescent cells preferentially expressed lymphoid precursor genes, while in actively cycling cells, the relative level of genes specific for individual lineages were differently correlated with stages of cell cycle. Granulocytic/monocytic and megakaryocytic precursor mRNA levels rose in early G1 phase, while erythroid specific expression showed selective augmentation at the G2/M phase. Stimulation of erythropoiesis shifted most HSCs into the active cell fraction without a disproportionate increase in the expression of early erythroid genes. Most transcription factors of the hematopoietic lineages were randomly expressed in a number of the early precursor cells but certain transcription factor mRNAs were detected only in very few cells. The latter genes may act as valves controlling the flow of differentiation. In summary we detect multi-level heterogeneity of the earliest hematopoietic precursor cells, demonstrate novel lineage specific cell cycle effects on the expression of precursor genes, and find that key regulatory genes for stages of differentiation can be identified from their silencing in stem cells.
 
Overall design RNA samples from HSC, MPPs (both from normal and bled mice) and the other early precursors were obtained. Gene expression of those cells were analyzed with single cell- and bulk RNA-seq.
 
Contributor(s) Yang J, Tanaka Y, Pan X, Seay M, Weissman SM
Citation(s) 28003475
Submission date May 18, 2015
Last update date Mar 21, 2019
Contact name Sherman Weissman
E-mail sherman.weissman@yale.edu
Phone 2037372281
Organization name Yale University
Department Genetics
Lab Sherman Weissman
Street address 1 Gilbert street
City New Haven
State/province CT
ZIP/Postal code 06519
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (786)
GSM1689161 HSC 1 rep1
GSM1689162 HSC 2 rep1
GSM1689163 HSC 3 rep1
Relations
BioProject PRJNA284255
SRA SRP058443

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68981_FPKM_table_PMA_RNA-seq.txt.gz 781.3 Kb (ftp)(http) TXT
GSE68981_FPKM_table_single-cell_RNA-seq.txt.gz 4.2 Mb (ftp)(http) TXT
GSE68981_FPKM_table_single-cell_RNA-seq_HSCspike.txt.gz 1.1 Mb (ftp)(http) TXT
GSE68981_FPKM_table_single-cell_RNA-seq_bledmice.txt.gz 2.1 Mb (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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