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Series GSE68899 Query DataSets for GSE68899
Status Public on May 15, 2015
Title Transcriptomic analysis of Clostridium acetobutylicum biofilm cells adsorbed onto fibrous matrix in biobutanol production [I]
Platform organisms Clostridium acetobutylicum; Arthrobacter sp.
Sample organism Clostridium acetobutylicum
Experiment type Expression profiling by array
Summary Immobilization of Clostridium acetobutylicum B3 onto fibrous matrix by surface-adsorption was developed and applied to biobutanol production. The immobilized C. acetobutylicum B3 cells formed biofilm and showed dramatically improved butanol tolerance and production rate. DNA array-based transcriptional analysis of C.acetobutylicum B3 biofilm cells was conducted to elucidate the gene expression profile of the biofilm cells. Results showed that about 16% of the whole genome was differentially expressed. The most apparently differentially expressed genes were involved in amino acid transport and metabolism, inorganic ion transport and metabolism, energy production and conversion, and coenzyme transport and metabolism.
 
Overall design Samples for biofilm cells and planktonic cells were withdrawn at four diffierent fermentation phases. The gene expression pattern of biofilm cells were investigated relative to that of planktonic cells from the same phase. The experiment was carried out twice independently. Cotton fibrous matrix (60 g/L) was used as biofilm carrier.
 
Contributor(s) Liu D, Xu J, Wang Y, Chen Y, Ying H
Citation(s) 26621081
Submission date May 14, 2015
Last update date Dec 04, 2015
Contact name Dong Liu
Organization name Nanjing Tech University
Department National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering
Lab Prof. Hanjie Ying
Street address No. 30, Puzhu South Road
City Nanjing
State/province Jiangsu
ZIP/Postal code 211816
Country China
 
Platforms (1)
GPL19013 Agilent-042658 Clostridium acetobutylicum ATCC 824 and Arthrobacte sp. CGMCC 3584 8X15K array [Probe Name Version]
Samples (8)
GSM1686164 Biofilm_phase_1_10h
GSM1686165 Biofilm_phase_2_15h
GSM1686166 Biofilm_phase_3_20h
This SubSeries is part of SuperSeries:
GSE68901 Transcriptomic analysis of Clostridium acetobutylicum biofilm cells adsorbed onto fibrous matrix in biobutanol production
Relations
BioProject PRJNA283992

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68899_RAW.tar 18.1 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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