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Series GSE6864

Query DataSets for GSE6864

Status

Public on Jul 04, 2007

Title

Gene expression regulation of transporters and phase I/II metabolic enzymes in murine small intestine during fasting

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Gene expression regulation of transporters and phase I/II metabolic enzymes in murine small intestine during fasting
Keywords: metabolic state analysis

Overall design

Pure bred wild-type (129S1/SvImJ) and PPARα-null (129S4/SvJae) mice were maintained on AIN93M diet, and subsequently two sets of mice were fasted for 24 hours. The complete intestines were then removed and total RNA was isolated.
In total 4 experimental groups were present: wild type and knockout mice that had free access to feed (AIN93M) before they were killed; wild type and knockout mice mice that were fasted for exactly 24 hours before they were killed.
RNA of 3 biological replicates was hybridized to Affymetrix MOE430A arrays. Five microgram total RNA was labelled according to the ENZO protocol, fragmented and hybridized according to Affymetrix's protocols.

Contributor(s)

van den Bosch HM, Bunger M, van der Meijde J, Hooiveld GJ, Muller M

Citation(s)

17683626

Submission date

Jan 25, 2007

Last update date

Jan 08, 2019

Contact name

Guido Hooiveld

E-mail(s)

guido.hooiveld@wur.nl