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Series GSE68544 Query DataSets for GSE68544
Status Public on Jun 15, 2015
Title Human brown and white preadipocyes clonal dynamics predict themorgenic potential and function
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Brown adipose tissue (BAT) holds therapeutic potential for obesity and metabolic syndrome via increasing energy expenditure. Both inter- and intra-individual differences contribute to heterogeneity in human BAT and potentially to differential thermogenic capacity in human populations. Here, we demonstrated the generation of brown and white preadipocyte clones from human neck fat and characterized their adipogenic differentiation and thermogenic function. Combining a UCP1 reporter system and gene expression profiling, we defined novel sets of gene signatures in human preadipocytes that could predict the thermogenic potential of mature adipocytes. Knocking out the positive UCP1 regulators, PREX1 and EDNRB, in brown preadipocytes by CRISPRs markedly abolished the high level of UCP1 in mature brown adipocytes. Finally, we showed the ability to prospectively isolate adipose progenitors with great thermogenic potential. These data provide new insights into the cellular heterogeneity in human fat and offer clinically relevant gene targets that mark thermogenically competent preadipocytes.
 
Overall design Highly adipogenic clonal white and brown cell lines
 
Contributor(s) Xue R, Lynes MD, Dreyfuss JM, Shamsi F, Schulz TJ, Zhang H, Huang TL, Townsend KL, Li Y, Takahashi H, Weiner LS, White AP, Lynes MS, Rubin LL, Goodyear LJ, Cypess AM, Tseng Y
Citation(s) 26076036
Submission date May 05, 2015
Last update date Aug 23, 2018
Contact name Jonathan M Dreyfuss
Organization name Joslin Diabetes Center
Street address 1 Joslin Pl
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL15207 [PrimeView] Affymetrix Human Gene Expression Array
Samples (41)
GSM1674867 clone1
GSM1674868 clone2
GSM1674869 clone3
Relations
BioProject PRJNA283051

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68544_RAW.tar 80.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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