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| Status |
Public on Apr 29, 2015 |
| Title |
Mouse granule cell neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A (microRNA) |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The purpose is to obtain samples for transcriptional analysis in triplicate wells using wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant virus (WNVE218A) in mouse granule cell neurons. This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently.
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| Overall design |
Granule cell neurons from day 6 C57Bl/6J mouse pups are infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) with multiplicity of infection (MOI) 250. Three technical replicates were performed at each of 1, 8, 12 and 24 hrs post infection. Time matched mocks done in triplicate are treated with mockulum: cell media concentrated through ultracentrifugation and diluted as virus. mRNA is sampled at all time points; microRNA is sampled at 12 hours post-infection. There were two independent biological replicates of the entire procedure, distinguished by sample name prefixes ('WGCN002' and 'WGCN003') and the biological_replicate characteristic field.
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| Contributor(s) |
Thackray L, Diamond M, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y |
| Citation(s) |
38565538 |
| |
| Submission date |
Apr 28, 2015 |
| Last update date |
Jun 19, 2024 |
| Contact name |
Natalie Heller |
| E-mail(s) |
natalie.heller@pnnl.gov
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| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
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| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
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| Platforms (1) |
| GPL19970 |
Agilent-046065 Mouse_miRNA_V19.0_Microarray [Probe Name version] |
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| Samples (17)
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| This SubSeries is part of SuperSeries: |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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| Relations |
| BioProject |
PRJNA282547 |
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