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Series GSE68381 Query DataSets for GSE68381
Status Public on Apr 29, 2015
Title Mouse granule cell neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A (microRNA)
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary The purpose is to obtain samples for transcriptional analysis in triplicate wells using wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant virus (WNVE218A) in mouse granule cell neurons. This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently.
Overall design Granule cell neurons from day 6 C57Bl/6J mouse pups are infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) with multiplicity of infection (MOI) 250. Three technical replicates were performed at each of 1, 8, 12 and 24 hrs post infection. Time matched mocks done in triplicate are treated with mockulum: cell media concentrated through ultracentrifugation and diluted as virus. mRNA is sampled at all time points; microRNA is sampled at 12 hours post-infection. There were two independent biological replicates of the entire procedure, distinguished by sample name prefixes ('WGCN002' and 'WGCN003') and the biological_replicate characteristic field.
Contributor(s) Thackray L, Diamond M, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y
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Submission date Apr 28, 2015
Last update date Aug 31, 2016
Contact name Natalie Heller
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
Platforms (1)
GPL19970 Agilent-046065 Mouse_miRNA_V19.0_Microarray [Probe Name version]
Samples (17)
GSM1670660 WGCN002_mock_12h_1_microRNA
GSM1670661 WGCN002_mock_12h_2_microRNA
GSM1670662 WGCN002_mock_12h_3_microRNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
BioProject PRJNA282547

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68381_RAW.tar 48.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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