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| Status |
Public on Jan 26, 2016 |
| Title |
Ambient O2 pressure induces NF-kB1/RelA related inflammatory response in human lung epithelial cells in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Oxygen (O2) levels in cell culture conditions is typically 2-5 fold higher than the physiological O2 levels that most tissues experience in vivo. The ambient atmospheric O2 (21%) is known to induce cell proliferation defects and cellular senescence in stem cell and primary cell cultures. Therefore, culturing these cells under lower O2 levels (2-9%) is currently a standard practice. However, the non-cancerous immortalized cells and cancer cells, which evade cellular senescence are normally cultured under 21% O2 levels and the effects of higher O2 levels on these cells are not fully understood.
Methods: Gene expression (RNA seq transcriptomics) analysis of immortalized human bronchial epithelial (BEAS-2B) cells cultured at ambient 21% O2 and lower 10% O2 levels for 3 days and 3 weeks. Further the beneficial effects of cuturing cells under lower oxygen tension is evalulated
Results: Our results show NF-κB/RelA mediated activation of pro-inflammatory cytokines as a major outcome of cells being cultured 21% O2. Moreover, we demonstrate increased RelA binding at the NF-κB1/RelA target gene promoters at 21% O2. Interestingly, contrary to cells cultutred at 21% O2, external stress induced by H2O2 exposure did not induce inflammatory response in cells grown at 10% O2, suggesting increased ability to handle external stress in cells cultured at lower O2 levels.
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| Overall design |
RNA Seq gene expression comparision done in replicates
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| Contributor(s) |
Cuddapah S, Coasta M, Jagannathan L |
| Citation(s) |
26588041 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 ES023174 |
Epigenetic Dysregulation by Oxidative Stress from Environmental Insults |
NEW YORK UNIVERSITY SCHOOL OF MEDICINE |
Cuddapah |
| R01 ES024727 |
Epigenetic reprogramming by nickel through chromatin domain disruption |
NEW YORK UNIVERSITY SCHOOL OF MEDICINE |
Cuddapah |
|
| Submission date |
Apr 28, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Suresh Cuddapah |
| E-mail(s) |
Suresh.Cuddapah@nyumc.org
|
| Organization name |
New York University Langone Medical center
|
| Department |
Environmental Sciecne
|
| Street address |
57 Old Forge Road
|
| City |
Tuxedo |
| State/province |
New York |
| ZIP/Postal code |
10987 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA282543 |
| SRA |
SRP057766 |
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