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Series GSE68131 Query DataSets for GSE68131
Status Public on Apr 22, 2015
Title Methylation Profile of postmature-green-stage seed and dry seed from Arabidopsis ddcc mutant
Organism Arabidopsis thaliana
Experiment type Methylation profiling by high throughput sequencing
Summary The role of non-CG methylation in seed development and dormancy remains unknown. There are four genes in charge of non-CG methylation in Arabidopsis: drm1, drm2, cmt2 and cmt3. The majority of non-CG methylation in vegetative tissues, leaf, is gone in homozygous ddcc mutant line (Hume et al., 2014). To uncover the possible role of non-CG DNA methylation in seed development and dormancy, we characterized the methylome of ddcc mutant in Arabidopsis postmature-green-stage seed and dry seed using Illumina sequencing. Meanwhile, vegetative tissue, leaves from 3 week plant with ddcc mutant and from wild-type, and postmature-green-stage seed and dry seed from wild-type plant were used as control.
 
Overall design Illumina sequencing of bisulfite-converted genomic DNA from postmature-green-stage seed, dry seed, and 3-week-plant leaves of ddcc mutant and wild-type.
 
Contributor(s) Goldberg RB, Lin J, Chen M
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Submission date Apr 21, 2015
Last update date Dec 23, 2018
Contact name Bob Goldberg
E-mail bobglab@mcdb.ucla.edu
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
Samples (6)
GSM1664376 ddcc mutant dry seed
GSM1664377 ddcc mutant leaf
GSM1664378 wild type dry seed
Relations
SRA SRP057531
BioProject PRJNA281884

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68131_RAW.tar 1017.0 Mb (http)(custom) TAR (of TXT)
Raw data are available in SRA
Processed data provided as supplementary file

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