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| Status |
Public on Jul 12, 2015 |
| Title |
Gene expression changes in MV4-11 cells upon SMARCA4 knock-down and PFI-3 treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Pharmacological inhibition of the SMARCA4 bromodomain inhibited human leukemic cell proliferation, phenocopying SMARCA4 knockdown in these cells.
We performed microarray analysis of global gene expression changes in MV4-11 cells after 6 days of PFI-3 treatment and after SMARCA4 knock-down. With this analysis we identified several genes whose expression was similarly up- or down-regulated upon inhibitor treatment and SMARCA4 depletion.
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| Overall design |
Human acute monocytic leukemia cells (MV4-11, ACC 102) were lentivirally transduced with shRNA taregting SMARCA4 (KD) or with a negative control shRNA (Scr). In a parallel experiment MV4-11 cells were treated for 6 days with 10μM PFI-3, which is SMARCA4, SMARCA2 and PBRM1 bromodomain inhibitor, or DMSO as a negative control. All sample types were prepared in triplicates.
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| Contributor(s) |
Cruickshank VA, Sroczynska P, Helin K |
| Citation(s) |
26571505 |
| Submission date |
Apr 15, 2015 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Kristian Helin |
| E-mail(s) |
kristian.helin@bric.ku.dk
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| Organization name |
Copenhagen University
|
| Department |
BRIC
|
| Street address |
Ole Maaløes vej 5
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE67924 |
Targeting of SMARCA4 bromodomain selectively inhibits leukemic cell proliferation |
|
| Relations |
| BioProject |
PRJNA281225 |
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