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Series GSE67920 Query DataSets for GSE67920
Status Public on Jul 12, 2015
Title Gene expression changes in MV4-11 cells upon SMARCA4 knock-down and PFI-3 treatment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Pharmacological inhibition of the SMARCA4 bromodomain inhibited human leukemic cell proliferation, phenocopying SMARCA4 knockdown in these cells.
We performed microarray analysis of global gene expression changes in MV4-11 cells after 6 days of PFI-3 treatment and after SMARCA4 knock-down. With this analysis we identified several genes whose expression was similarly up- or down-regulated upon inhibitor treatment and SMARCA4 depletion.
 
Overall design Human acute monocytic leukemia cells (MV4-11, ACC 102) were lentivirally transduced with shRNA taregting SMARCA4 (KD) or with a negative control shRNA (Scr). In a parallel experiment MV4-11 cells were treated for 6 days with 10μM PFI-3, which is SMARCA4, SMARCA2 and PBRM1 bromodomain inhibitor, or DMSO as a negative control. All sample types were prepared in triplicates.
 
Contributor(s) Cruickshank VA, Sroczynska P, Helin K
Citation(s) 26571505
Submission date Apr 15, 2015
Last update date Oct 29, 2019
Contact name Kristian Helin
E-mail(s) kristian.helin@bric.ku.dk
Organization name Copenhagen University
Department BRIC
Street address Ole Maaløes vej 5
City Copenhagen
ZIP/Postal code 2200
Country Denmark
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (12)
GSM1658531 SCR.1
GSM1658532 SCR.2
GSM1658533 SCR.3
This SubSeries is part of SuperSeries:
GSE67924 Targeting of SMARCA4 bromodomain selectively inhibits leukemic cell proliferation
Relations
BioProject PRJNA281225

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67920_RAW.tar 54.3 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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