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Series GSE67903 Query DataSets for GSE67903
Status Public on Mar 11, 2016
Title Genome-wide identification of CCA1-bound genes in Arabidopsis by ChIP-Seq
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary LHY and CCA1 encode single MYB transcription factors, involved in circadian clock. However, direct target genes of LHY and CCA1 in a genomic scale were largely unknown. To reveal bound genes by CCA1, chimeric protein CCA1-FLAG was expressed under CCA1 promoter in cca1 lhy (CCA1pro:CCA1-FLAG/ cca1 lhy). ChIP was performed using anti-FLAG antibody (F3165; SIGMA), which was bound to Dynabeads Protein G (100-03D; Life Technologies), and ChIP DNA were analyzed by IonPGM or Illumina GAII.
 
Overall design Chromatin immunoprecipitation was performed for CCA1-FLAG-expressing Arabidopsis. ChIP DNA was analyzed 2 types of deep sequencers (Illumina GAII and IonPGM).
 
Contributor(s) Nakamichi N, Suzuki T
Citation(s) 26941090
Submission date Apr 15, 2015
Last update date May 15, 2019
Contact name Norihito Nakamichi
E-mail(s) nnakamichi@itbm.nagoya-u.ac.jp
Phone +81-52-789-3656
Organization name Nagoya University
Department Institute of Transformative Bio-Molecules
Street address Furo-cho, Chikusa
City Nagoya
State/province Aichi
ZIP/Postal code 464-8602
Country Japan
 
Platforms (2)
GPL9302 Illumina Genome Analyzer II (Arabidopsis thaliana)
GPL19056 Ion Torrent PGM (Arabidopsis thaliana)
Samples (2)
GSM1657732 CCA1-ChIP_IonPGM
GSM1657733 CCA1-ChIP_Illumina
Relations
BioProject PRJNA281176
SRA SRP057254

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67903_RAW.tar 90.0 Kb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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