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Series GSE67875 Query DataSets for GSE67875
Status Public on Apr 03, 2016
Title A molecular shift in limb identity underlies the convergent evolution and development of feathered feet
Organism Columba livia
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The genetic and developmental mechanisms that control the decision between scale and feather growth – two profoundly different epidermal appendages, and an important developmental shift in the evolution of birds from their dinosaurian ancestors – remain poorly understood. Domestic pigeons display dramatic variation in foot epidermal appendages within a single species, and classical studies suggest that a small number of genes control much of this variation; thus pigeons provide a tractable model to understand skin appendage specification and variation. Here we show that feathered feet in pigeons are the consequence of a partial transformation of limb-type identity mediated by cis-regulatory changes in the hindlimb-specific transcription factor Pitx1 and forelimb-specific transcription factor Tbx5. We also demonstrate that ectopic hindlimb expression of Tbx5 is associated with the development of foot feathers in domestic chickens, suggesting that similar developmental mechanisms underlie phenotypic convergence in avian lineages that diverged over 100 MYA. These results show how qualitative and quantitative changes in expression of regional patterning genes can generate localized changes in organ fate and morphology, and provide a viable molecular mechanism for the evolution of hindlimb scale and feather distribution in dromaeosaurs.
Overall design Examination of H3K27ac status in embryonic limb buds from two domestic pigeon breeds, racing homer and Indian fantail
Contributor(s) Domyan ET, Shapiro MD, Infante CR, Menke DB
Citation(s) 26977633
Submission date Apr 14, 2015
Last update date May 15, 2019
Contact name Eric Domyan
Phone 801-863-5422
Organization name Utah Valley University
Department Department of Biology
Lab Domyan
Street address 800 W University Parkway
City Orem
State/province UT
ZIP/Postal code 84062
Country USA
Platforms (1)
GPL15807 Illumina HiSeq 2000 (Columba livia)
Samples (16)
GSM1657471 Homer_forelimb_input_DNA_Rep1
GSM1657472 Homer_forelimb_H3K27ac_ChIP_Rep1
GSM1657473 Homer_hindlimb_input_DNA_Rep1
BioProject PRJNA281098
SRA SRP057162

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67875_diff_indian_hindlimb_vs_homer_hindlimb_c3.0_cond1.bed.gz 721 b (ftp)(http) BED
GSE67875_diff_indian_hindlimb_vs_homer_hindlimb_c3.0_cond2.bed.gz 742 b (ftp)(http) BED 863.7 Mb (ftp)(http) BW
GSE67875_homer.forelimb_peaks.bed.gz 234.5 Kb (ftp)(http) BED 827.3 Mb (ftp)(http) BW
GSE67875_homer.hindlimb_peaks.bed.gz 267.5 Kb (ftp)(http) BED 834.1 Mb (ftp)(http) BW
GSE67875_indian.forelimb_peaks.bed.gz 219.1 Kb (ftp)(http) BED 968.7 Mb (ftp)(http) BW
GSE67875_indian.hindlimb_peaks.bed.gz 318.3 Kb (ftp)(http) BED
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Processed data are available on Series record

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