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Series GSE6784 Query DataSets for GSE6784
Status Public on Mar 01, 2007
Title Expression data from MCF10A cells subject to EGF stimulation
Organism Homo sapiens
Experiment type Expression profiling by array
Summary MCF10A cells derived from spontaneously immortalized normal human mammary epithel were subjected to EGF/SERUM stimulation for 0,20,40,60,120,240 and 480 minutes.
We used microarrays to understand the temporal regulation of the cellular EGFR cascade.
Keywords: time course
 
Overall design MCF10A cells were grown in DME:F12 medium supplemented with antibiotics, [10 mg/ml insulin, 0.1 mg/ml cholera toxin, 0.5 mg/ml hydrocortisone, heat-inactivated horse serum (5%); defined as 'serum' in the text] and 10ng/ml EGF. Cells were serum deprived for 24 hours. Following stimulation with EGF/SERUM for 0,20,40,60,120,240 and 480 minutes.
 
Contributor(s) Ido A, Ami C, Tal S, Eytan D, Yosef Y
Citation(s) 17322878
Submission date Jan 18, 2007
Last update date Dec 06, 2018
Contact name Tal Shay
Organization name Weizmann Institute of Science
Department Physics of Complex Systems
Street address Herzel
City Rehovot
ZIP/Postal code 26100
Country Israel
 
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (13)
GSM156771 Unstimulated MCF10A cells
GSM156772 20 MIN EGF STIMULATED MCF10A cells
GSM156773 40 MIN EGF STIMULATED MCF10A cells
This SubSeries is part of SuperSeries:
GSE6786 HeLa cells and MCF10A cells subject to EGF stimulation
Relations
BioProject PRJNA104123

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6784_RAW.tar 40.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file

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