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Status |
Public on May 20, 2015 |
Title |
A survey of human brain transcriptome diversity at the single cell level |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We used single cell RNA sequencing on 466 cells to capture the cellular complexity of the adult and fetal human brain at a whole transcriptome level. Healthy adult temporal lobe tissue was obtained from epileptic patients during temporal lobectomy for medically refractory seizures. We were able to classify individual cells into all of the major neuronal, glial, and vascular cell types in the brain.
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Overall design |
Examination of cell types in healthy human brain samples.
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Contributor(s) |
Darmanis S, Enge M, Quake SR, Sloan SA, Barres BA, Zhang Y, Caneda C, Hayden Gephart MG, Shuer LM |
Citation(s) |
26060301 |
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Submission date |
Apr 14, 2015 |
Last update date |
May 15, 2019 |
Contact name |
Martin Enge |
E-mail(s) |
martin.enge@ki.se
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Organization name |
Karolinska Institute
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Department |
Dep of Oncology-Pathology
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Street address |
CCK, Z4
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City |
Stockholm |
ZIP/Postal code |
S-171 76 |
Country |
Sweden |
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Platforms (2) |
GPL15520 |
Illumina MiSeq (Homo sapiens) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (466)
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Relations |
BioProject |
PRJNA281204 |
SRA |
SRP057196 |