|
| Status |
Public on Sep 20, 2016 |
| Title |
Single-cell RNA-seq of murine epidermis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used single-cell RNA sequencing to reveal how heterogeneity and plasticity of keratinocytes is tuned at the transcriptional level
|
| |
|
| Overall design |
We isolated and analyzed totally 1422 epidermal cells from dorsal telogen skin of C57BL/6 mice aged 56 +/-4 days. Sca1-microbeads were used to increase HF cell numbers and Sca1- (HF) and Sca1+ (mainly IFE) cell fractions were captured in a ratio of 2:1 on a microfluidic Fluidigm C1 system.
|
| |
|
| Contributor(s) |
Joost S, Zeisel A, Linnarsson S, Kasper M |
| Citation(s) |
27641957 |
| |
| Submission date |
Apr 06, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Sten Linnarsson |
| Organization name |
Karolinska Institutet
|
| Department |
Medical Biochemistry and Biophysics
|
| Lab |
Molecular Neurobiology
|
| Street address |
Scheeles väg 1
|
| City |
Stockholm |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (1422)
|
|
| Relations |
| BioProject |
PRJNA280413 |
| SRA |
SRP056902 |
Less...
More...