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Series GSE67543 Query DataSets for GSE67543
Status Public on Jun 08, 2015
Title Novel Observations from Next Generation RNA Sequencing of Highly Purified Human Adult and Fetal Islet Cell Subsets
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Understanding distinct gene expression patterns of normal adult and developing fetal human pancreatic a and b cells is crucial for developing stem cell therapies, islet regeneration strategies, and therapies designed to increase b cell function in patients with diabetes (type 1 or 2). Toward that end, we have developed methods to highly purify a, b, and d cells from human fetal and adult pancreata by intracellular staining for the cell-specific hormone content, sorting the sub-populations by flow cytometry and, using next generation RNA sequencing, we report on the detailed transcriptomes of fetal and adult a and b cells. We observed that human islet composition was not influenced by age, gender, or body mass index and transcripts for inflammatory gene products were noted in fetal b cells. In addition, within highly purified adult glucagon-expressing a cells, we observed surprisingly high insulin mRNA expression, but not insulin protein expression. This transcriptome analysis from highly purified islet a and b cell subsets from fetal and adult pancreata offers clear implications for strategies that seek to increase insulin expression in type 1 and type 2 diabetes.
 
Overall design RNA-sequencing of highly purified human adult and fetal islet cell subset was performed using our newly developed method. Using this data, we can study and compare the detailed transcriptome or alpha and beta cells during development.
 
Contributor(s) Blodgett DM, Nowosielska A, Afik S, Pechhold S, Cura AJ, Kennedy NJ, Kim S, Kucukural A, Davis RJ, Kent SC, Greiner DL, Garber MG, Harlan DM, diIorio P
Citation(s) 25931473
Submission date Apr 02, 2015
Last update date May 15, 2019
Contact name Shaked Afik
E-mail(s) shakedafik@gmail.com
Organization name UC Berkeley
Department Computational Biology Graduate Group
Street address Stanley Hall
City Berkeley
State/province CA
ZIP/Postal code 94720
Country USA
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (24)
GSM1649191 adult_beta_1
GSM1649192 adult_beta_2
GSM1649193 adult_beta_3
Relations
BioProject PRJNA280220
SRA SRP056835

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Supplementary file Size Download File type/resource
GSE67543_rna.seq.tpm.xlsx 4.0 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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