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Series GSE67511 Query DataSets for GSE67511
Status Public on Nov 30, 2015
Title Insights on cryoprotectant toxicity from gene expression profiling of endothelial cells exposed to ethylene glycol
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cryopreservation consists of preserving living cells or tissues at <-100˚C and has many applications in, for instance, stem cell and organ banking. Cryoprotectant agents, like ethylene glycol, are required for successful cryopreservation but have toxic side effects due to largely unknown mechanisms. In this work, we studied the toxicity of ethylene glycol in Human Umbilical Vein Endothelial Cells (HUVECs). Exposing cells to 60% ethylene glycol for two hours at 4˚C resulted in a slight decrease in cell growth, suggesting a modest toxicity of ethylene glycol and that HUVECs do not exhibit particular sensitivity to it. Gene expression analysis with whole genome micro-arrays revealed signatures indicative of a generalized stress response at 24 hours after stress and recovery at 72 hours, involving signaling pathways, glycoproteins, and genes involved in extracellular and transmembrane functions. These results reveal a new paradigm and signatures for future experiments in elucidating the toxicity effects of ethylene glycol in vascular endothelial cells.
Overall design 18 samples; 2 timepoints (24h and 72h), 6 controls per timepoint - 3 non treaded samples and 3 cold controls; other 3 were treated with increasing concentrations of EG.
Contributor(s) Cordeiro RM, Stirling S, Fahy G, Pedro de Magalhaes J
Citation(s) 26471925
Submission date Apr 01, 2015
Last update date Oct 29, 2018
Contact name Rui Augusto Martins Cordeiro
Organization name University of Liverpool
Department Integrative Biology
Lab Integrative Genomics of Ageing
Street address Crown Street
City Liverpool
State/province Merseyside
ZIP/Postal code L69 7ZB
Country United Kingdom
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (18)
GSM1648339 Huvecs, Untreated, 24h, Biological Rep 1
GSM1648340 Huvecs, Untreated, 24h, Biological Rep 2
GSM1648341 Huvecs, Untreated, 24h, Biological Rep 3
BioProject PRJNA280108

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Supplementary file Size Download File type/resource
GSE67511_RAW.tar 481.8 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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