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| Status |
Public on Nov 30, 2015 |
| Title |
Insights on cryoprotectant toxicity from gene expression profiling of endothelial cells exposed to ethylene glycol |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Cryopreservation consists of preserving living cells or tissues at <-100˚C and has many applications in, for instance, stem cell and organ banking. Cryoprotectant agents, like ethylene glycol, are required for successful cryopreservation but have toxic side effects due to largely unknown mechanisms. In this work, we studied the toxicity of ethylene glycol in Human Umbilical Vein Endothelial Cells (HUVECs). Exposing cells to 60% ethylene glycol for two hours at 4˚C resulted in a slight decrease in cell growth, suggesting a modest toxicity of ethylene glycol and that HUVECs do not exhibit particular sensitivity to it. Gene expression analysis with whole genome micro-arrays revealed signatures indicative of a generalized stress response at 24 hours after stress and recovery at 72 hours, involving signaling pathways, glycoproteins, and genes involved in extracellular and transmembrane functions. These results reveal a new paradigm and signatures for future experiments in elucidating the toxicity effects of ethylene glycol in vascular endothelial cells.
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| Overall design |
18 samples; 2 timepoints (24h and 72h), 6 controls per timepoint - 3 non treaded samples and 3 cold controls; other 3 were treated with increasing concentrations of EG.
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| Contributor(s) |
Cordeiro RM, Stirling S, Fahy G, Pedro de Magalhaes J |
| Citation(s) |
26471925 |
| Submission date |
Apr 01, 2015 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Rui Augusto Martins Cordeiro |
| E-mail |
ramcses@gmail.com
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| Organization name |
University of Liverpool
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| Department |
Integrative Biology
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| Lab |
Integrative Genomics of Ageing
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| Street address |
Crown Street
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| City |
Liverpool |
| State/province |
Merseyside |
| ZIP/Postal code |
L69 7ZB |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (18)
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| GSM1648339 |
Huvecs, Untreated, 24h, Biological Rep 1 |
| GSM1648340 |
Huvecs, Untreated, 24h, Biological Rep 2 |
| GSM1648341 |
Huvecs, Untreated, 24h, Biological Rep 3 |
| GSM1648342 |
Huvecs, Cold Control, 24h, Biological Rep1 |
| GSM1648343 |
Huvecs, Cold Control, 24h, Biological Rep2 |
| GSM1648344 |
Huvecs, Cold Control, 24h, Biological Rep3 |
| GSM1648345 |
Huvecs, EG treated, 24h, Biological Rep1 |
| GSM1648346 |
Huvecs, EG treated, 24h, Biological Rep2 |
| GSM1648347 |
Huvecs, EG treated, 24h, Biological Rep3 |
| GSM1648348 |
Huvecs, Untreated, 72h, Biological Rep 1 |
| GSM1648349 |
Huvecs, Untreated, 72h, Biological Rep 2 |
| GSM1648350 |
Huvecs, Untreated, 72h, Biological Rep 3 |
| GSM1648351 |
Huvecs, Cold Control, 72h, Biological Rep1 |
| GSM1648352 |
Huvecs, Cold Control, 72h, Biological Rep2 |
| GSM1648353 |
Huvecs, Cold Control, 72h, Biological Rep3 |
| GSM1648354 |
Huvecs, EG treated, 72h, Biological Rep1 |
| GSM1648355 |
Huvecs, EG treated, 72h, Biological Rep2 |
| GSM1648356 |
Huvecs, EG treated, 72h, Biological Rep3 |
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| Relations |
| BioProject |
PRJNA280108 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE67511_RAW.tar |
481.8 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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