NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE67474 Query DataSets for GSE67474
Status Public on Apr 04, 2015
Title Mouse cortical neuron transcriptional response to wild-type West Nile virus (WNV NY99 clone 382) and mutant virus WNV E218A [microRNA]
Organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary The purpose is to obtain samples for transcriptional analysis in triplicate wells using wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant virus (WNVE218A) in mouse cortical neurons. This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently.
 
Overall design Primary cortical neurons from C57Bl/6J mouse embryos (age E15) are infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) with multiplicity of infection (MOI) 250. Three technical replicates were performed at each of 1, 8, 12 and 24 hrs post infection. Time matched mocks done in triplicate are treated with mockulum: cell media concentrated through ultracentrifugation and diluted as virus. mRNA is sampled at all time points; microRNA is sampled at 12 hours post-infection. There were two independent biological replicates of the entire procedure, distinguished by sample name prefixes ('WCN002' and 'WCN003') and the biological_replicate characteristic field.
 
Contributor(s) Thackray L, Diamond M, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Mar 31, 2015
Last update date Aug 31, 2016
Contact name Natalie Heller
E-mail natalie.heller@pnnl.gov
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
 
Platforms (1)
GPL19970 Agilent-046065 Mouse_miRNA_V19.0_Microarray [Probe Name version]
Samples (16)
GSM1647803 WCN002_mock_12h_2_microRNA
GSM1647804 WCN002_mock_12h_3_microRNA
GSM1647805 WCN002_WNVWT_12h_1_microRNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
Relations
BioProject PRJNA280010

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67474_RAW.tar 45.7 Mb (http)(custom) TAR (of TXT)
GSE67474_normalized_data_with_miRNA_id.txt.gz 271.8 Kb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Processed data is available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap