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Series GSE67473 Query DataSets for GSE67473
Status Public on Apr 04, 2015
Title Mouse cortical neuron transcriptional response to wild-type West Nile virus (WNV-NY) and mutant virus WNV E218A [mRNA]
Organism Mus musculus
Experiment type Expression profiling by array
Summary The purpose is to obtain samples for transcriptional analysis in triplicate wells using wild type West Nile virus (WNV NY99 clone 382; WNVWT) and mutant virus (WNVE218A) in mouse cortical neurons. This data set comprises two complete biological replicate experiments conducted in the same conditions and with data processed independently.
Overall design Primary cortical neurons from C57Bl/6J mouse embryos (age E15) are infected with plasmid-derived wild type West Nile virus NY99 clone 382 (WNVWT) or plasmid-derived isogenic E218A mutant West Nile virus NY99 clone 382 (WNVE218A) with multiplicity of infection (MOI) 250. Three technical replicates were performed at each of 1, 8, 12 and 24 hrs post infection. Time matched mocks done in triplicate are treated with mockulum: cell media concentrated through ultracentrifugation and diluted as virus. mRNA is sampled at all time points; microRNA is sampled at 12 hours post-infection. There were two independent biological replicates of the entire procedure, distinguished by sample name prefixes ('WCN002' and 'WCN003') and the biological_replicate characteristic field.
Contributor(s) Thackray L, Diamond M, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y
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Submission date Mar 31, 2015
Last update date Nov 01, 2017
Contact name Natalie Heller
Organization name PNNL
Street address 902 Battelle Blvd.
City Richland
ZIP/Postal code 99354
Country USA
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (70)
GSM1647733 WCN002_mock_1h_1_mRNA
GSM1647734 WCN002_mock_1h_2_mRNA
GSM1647735 WCN002_mock_1h_3_mRNA
This SubSeries is part of SuperSeries:
GSE65575 Modeling Host Responses to Understand Severe Human Virus Infections
BioProject PRJNA280008

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67473_RAW.tar 150.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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