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Series GSE67259 Query DataSets for GSE67259
Status Public on Jul 20, 2015
Title Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells
Organisms Macaca fascicularis; Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Mechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell-like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRIN╬▒6. The transcriptomes of hPGCLCs and primordial germ cells (PGCs) isolated from non-human primates are similar, and although specification of hPGCLCs and mouse PGCs rely on similar signaling pathways, hPGCLC specification transcriptionally activates germline fate without transiently inducing eminent somatic programs. This includes genes important for naive pluripotency and repression of key epigenetic modifiers, concomitant with epigenetic reprogramming. Accordingly, BLIMP1, which represses somatic programs in mice, activates and stabilizes a germline transcriptional circuit and represses a default neuronal differentiation program. Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro.
 
Overall design RNAseq analysis of human induced pluripotent stem cells (hiPSC), incipient mesoderm-like cells (iMeLC) and primordial germ cell-like cells (BLIMP1-2A-tdTomato (BT)/TFAP2C-2A-ECFP (AG) expressing cells or EpCAM/CD49f (CSM) positive cells)
RNAseq analysis of mouse embryonic stem cells (mESC), epiblast-like cells (EpiLC) and primordial germ cell-like cells (PGCLC; Blimp1-mVenus (BV) or BV/stella-eCFP (SC) positive cells)
Single cell RNAseq analysis of cynomolgus ESCs and gonadal PGCs
 
Contributor(s) Sasaki K, Yokobayashi S, Nakamura T, Okamoto I, Yabuta Y, Kurimoto K, Ohta H, Moritoki Y, Iwatani C, Tsuchiya H, Sekiguchi K, Sakuma T, Yamamoto T, Mori T, Woltjen K, Nakagawa M, Yamamoto T, Takahashi K, Yamanaka S, Saitou M, Nakamura S
Citation(s) 26189426, 28649393
Submission date Mar 25, 2015
Last update date May 15, 2019
Contact name Yukihiro Yabuta
E-mail(s) yabyab@anat2.med.kyoto-u.ac.jp
Organization name Kyoto University, Graduate school of medicine
Department Anatomy and Cell Biology
Street address Yoshida-Konoe-cho, Sakyo-ku
City Kyoto
State/province Kyoto
ZIP/Postal code 606-8501
Country Japan
 
Platforms (3)
GPL15907 AB 5500xl Genetic Analyzer (Mus musculus)
GPL16288 AB 5500xl Genetic Analyzer (Homo sapiens)
GPL19944 AB 5500xl Genetic Analyzer (Macaca fascicularis)
Samples (74)
GSM1643143 hiPSC#1
GSM1643144 hiPSC#2
GSM1643145 iMeLC#1
Relations
BioProject PRJNA279357
SRA SRP056527

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67259_expression_cynomolgus.txt.gz 1.4 Mb (ftp)(http) TXT
GSE67259_expression_human.txt.gz 2.0 Mb (ftp)(http) TXT
GSE67259_expression_mouse.txt.gz 637.3 Kb (ftp)(http) TXT
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Processed data are available on Series record

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