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Series GSE67202 Query DataSets for GSE67202
Status Public on Jun 04, 2015
Title Transcriptomes of ΔrelAspoT and ΔdksA mutants of Haemophilus ducreyi in mid-log, transition, and stationary phase
Organism [Haemophilus] ducreyi 35000HP
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary To better understand the role of the (p)ppGpp-mediated stringent response in control of H. ducreyi virulence determinants, here we defined genes potentially regulated by either (p)ppGpp or DksA by using RNA-seq. We were able to show that loss of either (p)ppGpp or DksA resulted in dysregulation of multiple genes including several known virulence determinants. We also show that loss of (p)ppGpp or DksA resulted in differential expression of putative H. ducreyi small RNAs.
 
Overall design RNA of Haemophilus ducreyi wildtype, relAspoT and dksA mutants were collected at mid-log, transition, and stationary phases of growth, in quadruplicate using stranded RNA -seq.
 
Contributor(s) Holley C, Spinola SM
Citation(s) 26056381
Submission date Mar 24, 2015
Last update date May 15, 2019
Contact name Concerta L Holley
E-mail(s) clholley@iupui.edu
Organization name Indiana University School of Medicine
Department Microbiology and Immunology
Lab MS409D
Street address 635 Barnhill Drive
City Indianapolis
State/province IN
ZIP/Postal code 46202
Country USA
 
Platforms (1)
GPL19931 Illumina HiSeq 2500 (Haemophilus ducreyi 35000HP)
Samples (36)
GSM1642445 WT 4hr Rep 1
GSM1642446 WT 4hr Rep 2
GSM1642447 WT 4hr Rep 3
Relations
BioProject PRJNA279254
SRA SRP056479

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67202_Hducr_sRNA_differential_expression.xlsx 22.1 Kb (ftp)(http) XLSX
GSE67202_RSM_060514_Hducr_gene_differential_expression.xlsx 1.1 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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