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| Status |
Public on Dec 28, 2015 |
| Title |
RNAseq Analysis of Transcriptome expression profiles in E13.5 Foxf2-/-Osr2RFP/+ and control palatal mesenchymal cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The purpose of this study is to compare the transcriptome expression profiles of E13.5 Foxf2-/-;Osr2RFP/+ and control palatal mesenchyme by using RNA-seq analysis.
Methods: Foxf2+/- female mice were crossed with Foxf2+/-;Osr2RFP/+ male mice.The embryos were harvested at E13.5. The pair of palatal shelves were dissected from each Osr2-RFP+ embryo. The RFP+ palatal mesenchyme cells were isolated by using fluorescence-activated cell sorting (FACS). RNA-seq analysis was carried out using the FACS-isolated palatal mesenchyme from Foxf2-/-;Osr2RFP/+, Foxf2+/-;Osr2RFP/+ and Osr2RFP/+embryos, respectively.
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| Overall design |
The transcriptome expression profiles of E13.5 control and Foxf2-/-Osr2RFP/+ palatal mesenchyme by using RNA-seq analysis, in duplicates, using Illumina HisEq 2000.
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| Contributor(s) |
Xu J, Jiang R |
| Citation(s) |
26745863, 33898467 |
| Submission date |
Mar 18, 2015 |
| Last update date |
Apr 28, 2021 |
| Contact name |
Jingyue Xu |
| E-mail(s) |
jingyue.xu@cchmc.org
|
| Organization name |
Cincinnati Children's Hospital Medical Center
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| Department |
Developmental Biology
|
| Street address |
3333 burnet avenue
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45208 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA278803 |
| SRA |
SRP056312 |
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