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Series GSE67009 Query DataSets for GSE67009
Status Public on Nov 04, 2015
Title ESRP2 Regulates A Conserved And Cell-Type-Specific Splicing Program to Support Postnatal Liver Maturation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Alternative splicing greatly expands the proteomic diversity but its functional impact is often unclear. Here, we identify a highly conserved and temporally coordinated cell-type-specific splicing program, which is activated in part by ESRP2 during postnatal liver development. Consistent with failure of many neonatal-to-adult splicing transitions, Esrp2 null mice exhibit persistent expression of fetal markers and loss of mature hepatocyte characteristics. Conversely, ectopic expression of ESRP2 in immature mouse or human hepatocytes results in a reciprocal switch in splicing. Our findings define an essential role for ESRP2 in generation of conserved repertoires of adult splice isoforms that facilitate postnatal liver maturation.
Overall design Mouse liver RNA was isolated with Trizol (Invitrogen). Hi-Seq libraries were prepared and paired-end 100bp Illumina sequencing was performed on mouse liver samples from different developmental stages.
Contributor(s) Kalsotra A, Xiao X
Citation(s) 26531099
Submission date Mar 17, 2015
Last update date May 15, 2019
Contact name Jaegyoon Ahn
Organization name UCLA
Department IBP
Lab Xiao lab
Street address 611 Charles E. Young Drive
City LA
State/province CA
ZIP/Postal code 90095-1570
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (8)
GSM1635948 E18_rep1
GSM1635949 E18_rep2
GSM1635950 PN14_rep1
BioProject PRJNA278684
SRA SRP056267

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE67009_RAW.tar 2.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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