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Series GSE6696 Query DataSets for GSE6696
Status Public on Sep 08, 2008
Title Transcriptome analyses show changes in gene expression to accompany pollen germination and tube growth in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Pollen germination, along with pollen tube growth, is an essential process for the reproduction of flowering plants. The germinating pollen with tip-growth characteristics provides an ideal model system for the study of cell growth and morphogenesis. As an essential step towards a detailed understanding of this important process, the objective of this study was to comprehensively analyze the transcriptome changes during pollen germination and pollen tube growth. Using Affymetrix Arabidopsis ATH1 Genome Arrays, this study is the first to show the changes in the transcriptome from desiccated mature pollen grains to hydrated pollen grains and then to pollen tubes of Arabidopsis thaliana. The number of expressed genes, either for total expressed genes or for specifically expressed genes, increased significantly from desiccated mature pollen to hydrated pollen and again to growing pollen tubes, which is consistent with the finding that pollen germination and tube growth was significantly inhibited in vitro by a transcriptional inhibitor. The results of GO analyses showed that expression of genes related to cell rescue, transcription, signal transduction and cellular transport were significantly changed, especially for up-regulation, during pollen germination and tube growth, respectively. In particular, genes of the CaM/CML, CHX and Hsp families showed the most significant changes during pollen germination and tube growth. These results demonstrate that the overall transcription of genes, both in the number of expressed genes and in the levels of transcription, was increased. Furthermore, the appearance of many novel transcripts during pollen germination as well as tube growth indicates that these newly expressed genes may function in this complex process.
 
Overall design Three samples are analyzed in this experiment. They are desiccated mature pollen grains (MP), hydrated pollen grains (HP) and growing pollen tubes (PT) of Arabidopsis thaliana, respectively. Each sample has two biological replicates, so that there are 6 data sets of ATH1 array in this experiment.
 
Contributor(s) Wang Y, Zhang W, Song L, Zou J, Su Z, Wu W
Citation(s) 18775970
Submission date Jan 10, 2007
Last update date Aug 28, 2018
Contact name Wei-Hua Wu
Organization name China Agricultural University
Department College of Biological Sciences
Lab Wu Wei-Hua's Lab
Street address No.2 Yuanmingyuan West Road
City Beijing
State/province Beijing
ZIP/Postal code 100094
Country China
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (6)
GSM154503 Arabidopsis desiccated mature pollen grains rep1
GSM154504 Arabidopsis desiccated mature pollen grains rep2
GSM154505 Arabidopsis hydrated pollen grains rep1
Relations
BioProject PRJNA99003

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6696_RAW.tar 11.1 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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