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Series GSE66936 Query DataSets for GSE66936
Status Public on Mar 17, 2015
Title Expression data from intermediate monocytes from healthy donors and autoimmune uveitis patients
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human peripheral monocytes have been categorized into three subsets based on differential expression levels of CD14 and CD16. However, the factors that influence the distribution of monocyte subsets and the roles which each subset plays in autoimmunity are not well studied. To compare the gene expression profiling 1) on intermediate monocytes CD14++CD16+ monocytes between healthy donors and autoimmune uveitis patients and 2) among 3 monocyte subsets in health donors, here we purified circulating intermediate CD14++CD16+ monocytes from 5 patients with autoimmune uveitis (labeled as P1-5) and 4 healthy donors (labeled as HD1-4) by flow cytometry and isolated total RNA to proceed microarray assay. In addition, we also purified CD14+CD16++ (non-classical monocytes) and CD14++CD16- (classical monocytes) from 4 healthy donors to do microarray. We demonstrate that CD14++CD16+ monocytes from patients and healthy control donors share a similar gene expression profile. The CD14+CD16++ cells (non-classical monocytes) display the most distinctive gene expression profiling when compared to intermediate CD14++CD16+ monocytes and classical CD14++CD16- monocytes.
 
Overall design Pheripharal blood from 5 autoimmune uveitis patients and 4 healthy donors were collected. Immediately after collection, we purified circulating intermediate CD14++CD16+ monocytes from 5 patients with autoimmune uveitis ( Sample 13-17; titled as P intermediate 1-5) and 4 healthy donors (Sample 1, 4, 7, 10; titled as HD intermediate 1-4) by flow cytometry and isolated total RNA to proceed microarray assay. In addition, we also purified CD14+CD16++ (Sample 3, 6, 9, 12; titled HD non-classical 1-4) and CD14++CD16- (Sample 2, 5, 8, 11; titled HD classical 1-4) from 4 healthy donors to do microarray.

Please note that data processing was performed in two groups;
[1] HD intermediate, classical and non-classical samples (total 12 samples)
[2] HD intermediate samples reanalyzed with P intermediate samples (total 9 samples).
 
Contributor(s) Liu B
Citation(s) 25911752
Submission date Mar 16, 2015
Last update date Mar 25, 2019
Contact name Baoying Liu
E-mail(s) liuba@nei.nih.gov
Phone 301-594-5197
Organization name NEI
Street address 9000 Rockville Pike
City Bethesda
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (21)
GSM1634594 HD intermediate 1
GSM1634595 HD classical 1
GSM1634596 HD non-classical 1
Relations
BioProject PRJNA278404

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE66936_RAW.tar 157.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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