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Series GSE6689 Query DataSets for GSE6689
Status Public on Jan 07, 2008
Title Expression data during stem cell differentiation
Organism Mus musculus
Experiment type Expression profiling by array
Summary Stem cell development requires selection of specific genetic programs to direct cellular fate. Using microarray technology, we profile expression trends at selected timepoints during stem cell differentiation to characterize these changes.
Keyword(s): timecourse
 
Overall design Total RNA was isolated using the Micro-to-Midi isolation kit (Invitrogen), and subjected to comparative gene expression profiling by labelled cRNA hybridization to the mouse genome 430 2.0 microarray (Affymetrix). Data acquired using the GeneChip Scanner 3000 was analyzed with the Genespring GX 7.3 microarray data software bioinformatics suite (Agilent Technologies) restricting the derived gene list to identify differentially expressed genes defined by a >1.5-fold difference and P<0.05. Data population sets were normalized to the undifferentiated phenotype, and quality filtered to eliminate background noise prior to hierarchical clustering.
 
Contributor(s) Behfar A, Perez-Terzic C, Faustino RS, Arrell DK, Hodgson D, Yamada S, Puceat M, Niederlander N, Alekseev A, Zingman L, Terzic A
Citation(s) 18184438
Submission date Jan 09, 2007
Last update date Feb 11, 2019
Contact name Atta Behfar
E-mail(s) behfar.atta@mayo.edu
Organization name Mayo Clinic
Street address 200 First Street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (12)
GSM154465 Control_ES-LIF(+)_rep1
GSM154466 Timepoint1_ES-LIF(-)_rep1
GSM154467 Timepoint2_CM_rep1
Relations
BioProject PRJNA99069

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6689_RAW.tar 81.5 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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