|
| Status |
Public on May 16, 2007 |
| Title |
Nucleosome occupancy |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Chromatin plays roles in processes governed by different time scales. To assay the dynamic behaviour of chromatin in living cells, we used genomic tiling arrays to measure histone H3 turnover in G1-arrested S. cerevisiae at single-nucleosome resolution over 4% of the genome, and over the entire genome at lower (~265 bp) resolution. We find that nucleosomes at promoters are replaced more rapidly than at coding regions, and that replacement rates over coding regions correlate with polymerase density. In addition, rapid histone turnover is found at known chromatin boundary elements. These results suggest that rapid histone turnover serves to functionally separate chromatin domains and prevent spread of histone states.
Keywords: MNase, Nucleosomes
|
| |
|
| Overall design |
Nucleosomal vs genomic DNA.
|
| |
|
| Contributor(s) |
Dion MF, Kaplan T, Kim M, Buratowski S, Friedman N, Rando OJ |
| Citation(s) |
17347438 |
| |
| Submission date |
Jan 06, 2007 |
| Last update date |
Feb 15, 2018 |
| Contact name |
Tommy Kaplan |
| E-mail(s) |
tommy@cs.huji.ac.il
|
| Organization name |
Hebrew University
|
| Department |
School of Computer Science and Engineering
|
| Street address |
Givat Ram Campus
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL4131 |
Agilent-014810 Yeast Whole Genome ChIP-on-Chip Microarray 4x44K (G4493A) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
| GSE6680 |
Dynamics of replication-independent histone turnover in budding yeast |
|
| Relations |
| BioProject |
PRJNA104259 |
