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Series GSE66507 Query DataSets for GSE66507
Status Public on Aug 26, 2015
Title Single-Cell RNA-seq Defines the Three Cell Lineages of the Human Blastocyst
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Here we provide fundamental insights into early human development by single-cell RNA-sequencing of human and mouse preimplantation embryos. We elucidate conserved transcriptional programs along with those that are human-specific. Importantly, we validate our RNA-sequencing findings at the protein level, which further reveals differences in human and mouse embryo gene expression. For example, we identify several genes exclusively expressed in the human pluripotent epiblast including the transcription factor KLF17. Key components of the TGF-β signaling pathway including NODAL, GDF3, TGFBR1/ALK5, LEFTY1, SMAD2, SMAD4 and TDGF1 are also enriched in the human epiblast. Intriguingly, inhibition of TGF-β signaling abrogates NANOG expression in human epiblast cells, consistent with a requirement for this pathway in pluripotency. Although key trophectoderm factors Id2, Elf5, and Eomes are exclusively localized to this lineage in the mouse, the human orthologues are either absent or expressed in alternative lineages. Importantly, we also identify genes with conserved expression dynamics including Foxa2/FOXA2, which we show is restricted to the primitive endoderm in both human and mouse embryos. Comparisons of the human epiblast to existing embryonic stem cells (hESCs) reveals conservation of pluripotency but also additional pathways more enriched in hESCs. Our analysis highlights significant differences in human preimplantation development compared to mouse and provides a molecular blueprint to understand human embryogenesis and its relationship to stem cells.
 
Overall design Single-Cell RNA-seq
 
Contributor(s) Blakeley P, Niakan K
Citation(s) 26293300, 32968278
Submission date Mar 04, 2015
Last update date Nov 20, 2020
Contact name paul blakeley
E-mail(s) blakeleyp@gmail.com
Organization name Crick Institute
Street address 215 Euston Road
City London
ZIP/Postal code NW12BE
Country United Kingdom
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (30)
GSM1624222 TE8.4
GSM1624223 TE3.5
GSM1624224 TE3.1
Relations
BioProject PRJNA277181
SRA SRP055810

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SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE66507_human_blastocyst_rnaseq_counts.txt.gz 931.3 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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