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| Status |
Public on May 05, 2015 |
| Title |
Identification of N6-adenine methylation in C. elegans DNA |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Methylation profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In mammalian cells, DNA methylation on the 5th position of cytosine (5mC) plays an important role as an epigenetic mark. However, DNA methylation was considered to be absent in C. elegans because of the lack of detectable 5mC as well as homologs of the cytosine DNA methyltransferases. Here, using multiple approaches, we demonstrate the presence of adenine N6-methylation (6mA) in C. elegans DNA. We further demonstrate that this modification increases trans-generationally in a paradigm of epigenetic inheritance. Importantly, we identify a DNA demethylase, NMAD-1, and a potential DNA methyltransferase, DAMT-1, which regulate 6mA levels and crosstalk between methylation of histone H3K4me2 and 6mA, and control the epigenetic inheritance of phenotypes associated with the loss of the H3K4me2 demethylase spr-5. Together, these data identify a novel DNA modification in C. elegans and raise the exciting possibility that 6mA may be a carrier of heritable epigenetic information in eukaryotes.
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| Overall design |
SMRT-sequencing for a mixed cell population of wildtype worms
6mA ChIP-Seq for a mixed cell population of wildtype worms
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| Contributor(s) |
Gu L, Greer EL, Blanco MA, Sendinc E, Liu J, Aristizabal-Corrales D, Hsu C, Aravind L, He C, Shi Y |
| Citation(s) |
25936839, 31159718 |
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| Submission date |
Mar 04, 2015 |
| Last update date |
Jun 16, 2019 |
| Contact name |
Lei Gu |
| E-mail(s) |
lei.gu@mpi-bn.mpg.de
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| Organization name |
Max Planck Institute for heart and lung research
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| Street address |
parkstreet 1
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| City |
Bad Nauheim |
| State/province |
MASSACHUSETTS |
| ZIP/Postal code |
61231 |
| Country |
Germany |
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| Platforms (2) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
| GPL19919 |
PacBio RS II (Caenorhabditis elegans) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA278967 |
| SRA |
SRP056370 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66504_RAW.tar |
341.4 Mb |
(http)(custom) |
TAR (of BW, GFF, NARROWPEAK) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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