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| Status |
Public on Feb 26, 2016 |
| Title |
Expression data from differentiating mouse embryonic stem cells wild type and lacking functional Pax7 gene |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Myogenic differentiation relies on Pax7 function. We used embryonic stem cells lacking functional Pax7 to follow its role in derivation of skeletal myoblasts.
Microarray analysis allowed us to compare transcriptomes of undifferentiated and differentiating embryonic stem cells of two genotypes, i.e. Pax7+/+ and Pax7-/- at day 7 and 21 of culture.
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| Overall design |
Undifferentiated mouse embryonic stem cells, embryiod bodies at day 7, and embryoid body outgrowths at day 21 of differentiation were collected for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Czerwinska AM, Fogtman A, Grabowska I, Archacka K, Bem J, Iwanicka R, Koblowska M, Ciemerych MA |
| Citation(s) |
26649785 |
| Submission date |
Mar 03, 2015 |
| Last update date |
Mar 08, 2018 |
| Contact name |
Areta Magda Czerwinska |
| Organization name |
University of Warsaw
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| Department |
Faculty of Biology
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| Lab |
Department of Cytology
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| Street address |
Krakowskie Przedmiescie 26/28
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| City |
Warsaw |
| ZIP/Postal code |
00-096 |
| Country |
Poland |
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| Platforms (1) |
| GPL17400 |
[MoGene-2_1-st] Affymetrix Mouse Gene 2.1 ST Array [transcript (gene) version] |
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| Samples (18)
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| GSM1623171 |
Pax7+/+ ESCs day 7, biological rep 1 |
| GSM1623172 |
Pax7+/+ ESCs day 7, biological rep 2 |
| GSM1623173 |
Pax7+/+ ESCs day 7, biological rep 3 |
| GSM1623174 |
Pax7+/+ ESCs day 21, biological rep 1 |
| GSM1623175 |
Pax7+/+ ESCs day 21, biological rep 2 |
| GSM1623176 |
Pax7+/+ ESCs day 21, biological rep 3 |
| GSM1623177 |
Pax7-/- ESCs day 0, biological rep 1 |
| GSM1623178 |
Pax7-/- ESCs day 0, biological rep 2 |
| GSM1623179 |
Pax7-/- ESCs day 0, biological rep 3 |
| GSM1623180 |
Pax7-/- ESCs day 7, biological rep 1 |
| GSM1623181 |
Pax7-/- ESCs day 7, biological rep 2 |
| GSM1623182 |
Pax7-/- ESCs day 7, biological rep 3 |
| GSM1623183 |
Pax7-/- ESCs day 21, biological rep 1 |
| GSM1623184 |
Pax7-/- ESCs day 21, biological rep 2 |
| GSM1623185 |
Pax7-/- ESCs day 21, biological rep 3 |
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| Relations |
| BioProject |
PRJNA277047 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66483_RAW.tar |
90.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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