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Series GSE66328 Query DataSets for GSE66328
Status Public on Apr 29, 2015
Title s54 -dependent response to nitrogen limitation and virulence in Burkholderia cenocepacia H111
Organism Burkholderia cenocepacia H111
Experiment type Expression profiling by high throughput sequencing
Summary Members of the genus Burkholderia are versatile bacteria capable of colonizing highly diverse environmental niches. In this study, we investigated the global response of the opportunistic pathogen Burkholderia cenocepacia H111 to nitrogen limitation at the transcript and protein expression level. In addition to a classical response to nitrogen starvation, including the activation of glutamine synthetase, PII proteins and the two component regulatory system ntrBC, B. cenocepacia H111 also up-regulated polyhydroxybutyrate (PHB) accumulation and exopolysaccharide (EPS) production in response to nitrogen shortage. A search for consensus sequences in promoter regions of nitrogen responsive genes identified a s54 consensus sequence. The mapping of the s54 regulon as well as the characterization of a s54 mutant suggests an important role of s54 not only in control of nitrogen metabolism, but also in virulence of this organism.
Overall design Nitrogen limitation and s54 regulon in B. cenocepacia
Contributor(s) Lardi M, Aguilar C, Pedrioli A, Suppiger A, Cárcamo-Oyarce G, Schmid N, Omasits U, Ahrens CH, Eberl L, Pessi G
Citation(s) 25841012
Submission date Feb 26, 2015
Last update date May 15, 2019
Contact name Gabriella Pessi
Organization name University of Zürich
Street address Winterthurerstrasse 190
City Zürich
ZIP/Postal code 8057
Country Switzerland
Platforms (1)
GPL16128 Illumina HiSeq 2000 (Burkholderia cenocepacia H111)
Samples (5)
GSM1619836 H111 wt nitrogen replete
GSM1619837 H111 wt nitrogen starved
GSM1619838 H111 wt #2
BioProject PRJNA276555
SRA SRP055546

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Supplementary file Size Download File type/resource
GSE66328_RAW.tar 850.0 Kb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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